Human EGF Protein (EGF-HE001)
Catalogue Number: EGF-HE001-KAT
EGF-HE001 Image
EGF-HE001 Image
EGF-HE001 Image
EGF-HE001 Image
EGF-HE001 Image
EGF-HE001 Image
The purity of Human EGF is greater than 95% as determined by SEC-HPLC.
EGF-HE001 Image
Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, No Tag with an affinity constant of 1.33 nM as determined in SPR assay (Biacore T200).
EGF-HE001 Image
Human EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
EGF-HE001 Image
Immobilized Human EGF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 1.56ug/ml determined by ELISA (QC Test).
EGF-HE001 Image
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50-for this effect is typically 0.1 - 0.2 ng/mL (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 5.6 kDa same as Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Growth Factors |
| Alias: | HOMG4; EGF; URG |
| Host Cell: | E.coli |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
Additional Text
Protein Tag
Untagged
Short Description
Recombinant Human EGF Protein is expressed from E.coli without tag.It contains Asn971-Arg1023.
UniProt ID
P01133-1
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human EGF, No Tag at 2Oeg/ml (100Oel/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 1.3Oeg/ml determined by ELISA. The affinity constant of 0.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 0.2 EU per µg by the LAL method.
EGF-HE001 Datasheet
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