Biotinylated Human EGFR/HER1 Protein (EGF-HM401B)
Catalogue Number: EGF-HM401B-KAT
EGF-HM401B Image
EGF-HM401B Image
EGF-HM401B Image
EGF-HM401B Image
EGF-HM401B Image
Biotinylated Human EGFR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human EGF, No Tag with an affinity constant of 0.62 nM as determined in SPR assay (Biacore T200).
EGF-HM401B Image
The purity of Biotinylated Human EGFR is greater than 95% as determined by SEC-HPLC.
EGF-HM401B Image
Biotinylated Human EGFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
EGF-HM401B Image
Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 71.5 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Protein Kinases |
| Alias: | ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Additional Text
Protein Tag
HIS-AviTag™
Uniprot ID
P00533-1
Short Description
Recombinant Biotinylated Human EGFR/HER1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu25-Ser645.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
The affinity constant of 0.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
EGF-HM401B Datasheet
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