Catalogue Number: EN-176L-JEN
Manufacturer: | Jena Bioscience |
Shelf Life: | 12 months |
Molecular Weight: | 27.0 kDa |
Physical state: | Liquid |
Type: | Ribonucleases (RNase) |
Shipping Condition: | Blue Ice |
Unit(s): | 5 x 2000 units |
Description: Ribonuclease l (27 kD) is a completely nonspecific ribonuclease that hydrolyzes the phosphodiester bond of all four bases. It degrades any RNA to a mixture of mono-, di-, and trinucleotides and does not degrade DNA, although it does bind to DNA. It has a marked preference for single-stranded RNA over double-stranded RNA, which allows it to work well in RNase Protection Assays. It has a high specific activity which, coupled with its non-specificity, typically results in complete degradation of RNA using ng amounts of protein. Contains no endonuclease or exonuclease activity toward DNA substrates.
degrades RNA to cyclic nucleotide monophosphates leaving a 5'-OH and 2'-, 3'-cyclic monophosphateRibonuclease Protection AssaysMapping or Quantitation of RNA by selective cleavage of single-strand regionsEliminates RNA from DNA- and protein purification