RNase I (DNase free), Endonuclease, recombinant, E. coli
Catalogue Number: EN-176S-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Molecular Weight: | 27.0 kDa |
| Physical state: | Liquid |
| Type: | Ribonucleases (RNase) |
| Shipping Condition: | Blue Ice |
| Unit(s): | 2000 units |
Description
Description: Ribonuclease l (27 kD) is a completely nonspecific ribonuclease that hydrolyzes the phosphodiester bond of all four bases. It degrades any RNA to a mixture of mono-, di-, and trinucleotides and does not degrade DNA, although it does bind to DNA. It has a marked preference for single-stranded RNA over double-stranded RNA, which allows it to work well in RNase Protection Assays. It has a high specific activity which, coupled with its non-specificity, typically results in complete degradation of RNA using ng amounts of protein. Contains no endonuclease or exonuclease activity toward DNA substrates.
Additional Text
Application Notes
degrades RNA to cyclic nucleotide monophosphates leaving a 5'-OH and 2'-, 3'-cyclic monophosphateRibonuclease Protection AssaysMapping or Quantitation of RNA by selective cleavage of single-strand regionsEliminates RNA from DNA- and protein purification
EN-176S MSDS
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