Exonuclease I, recombinant, E. coli
Catalogue Number: EN-177S-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Physical state: | Liquid |
| Type: | Nucleases |
| Shipping Condition: | Blue Ice |
| Unit(s): | 2000 units |
Description
Description: Catalyses removal of nucleotides from single-stranded DNA in 3' -> 5' direction. 10x Reaction Buffer: 670 mM Glycine-KOH (pH 9.5 at 25 °C), 100 mM 2-mercaptoethanol and 67 mM MgCl2. Assay Conditions: 67 mM Glycin-KOH (pH 9.5 at 25 °C), 10 mM 2-mercaptoethanol, 6.7 mM MgCl2, 0.17 mg/ml single-stranded [3H]-DNA. Incubation is at 37 °C for 10 min in a reaction volume of 50 µl.
Additional Text
Concentration
20 unit/ul
Application Notes
removal of residual ssDNA, including oligonucleotides, from reaction mixturesdoes not degrade double-stranded DNA or RNArequires magnesium and presence of free, accessible 3'-hydroxyl-terminiactive in a wide variety of buffer conditions, allowing for direct addition of enzyme to most reaction mixtures
EN-177S MSDS
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