Catalogue Number: EN-181-1G-JEN
Manufacturer: | Jena Bioscience |
Shelf Life: | 6 months |
Molecular Weight: | 28.9 kDa |
Physical state: | Powder |
Type: | Proteases |
Alias: | Endopeptidase K |
Shipping Condition: | Blue Ice |
Unit(s): | 5 x 200 mg |
Description: Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The Protein with a molecular weight of 28.9 kDa cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinse K is not inactivated by metal chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4 - 12.5). Proteinase K is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF. In presence of 0.5 - 1 % SDS Proteinase K inactivates DNases and RNases in eucaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids.
#GHS08#
Digestion of proteins during DNA and RNA preparation.
39450-01-6