Biotinylated Human E-Selectin/CD62E Protein (ESE-HM401B)
Catalogue Number: ESE-HM401B-KAT
ESE-HM401B Image
ESE-HM401B Image
ESE-HM401B Image
ESE-HM401B Image
The purity of Biotinylated Human E-selectin is greater than 95% as determined by SEC-HPLC.
ESE-HM401B Image
Biotinylated Human E-selectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
ESE-HM401B Image
Immobilized Biotinylated Human E-selectin, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Protein - Conjugated |
| Alias: | CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E; RP1-117P20.2 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Additional Text
Gene Name
SELE
Gene ID
6401
Uniprot ID
P16581
Protein Tag
HIS-AviTag™
Short Description
Recombinant Biotinylated Human E-Selectin/CD62E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp22-Pro556.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Anti-E-Selectin Antibody at 2Oeg/ml (100Oel/well) on the plate. Dose response curve for Biotinylated Human E-selectin, His Tag with the EC50 of 78.4ng/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
ESE-HM401B Datasheet
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