Anti-Mouse FOXP3 [MF-14] - Purified (PhenoCyler-Fusion (CODEX)® Ready)

Catalogue Number: F405-LET

Manufacturer:	Leinco Technologies, Inc
Type:	Monoclonal Primary Antibody - Unconjugated
Shipping Condition:	Blue Ice
Unit(s):	50 ug
Host name:
Clone:	MF-14
Isotype:
Immunogen:
Application:	FC, ICC, IF, WB, Img

Overview

Description

Description: FOXP3 is a transcription factor1 required for development of CD4+CD25+ regulatory T cells (Treg) that does not affect CD4+CD25- T cell proliferation2. Treg cells expressing FOXP3 play a critical role in the maintenance of immune homeostasis and prevention of autoimmunity1. FOXP3 promotes the generation of Tregs, represses cytokine expression in primary CD4+ T cells, and suppresses the transcriptional activities of NFAT and NF-κB3. FOXP3 also inhibits basal activation of PKC-θ, IκB kinase β (IKKβ), and TNF-α stimulation of NF-κB. Depletion of FOXP3+CD4+ Treg cells and/or loss of function mutations of FOXP3 results in severe autoimmunity, allergy, immunopathology as well as cancer immunity1. FOXP3 is predominantly expressed in CD25+CD4+ Tregs in the thymus and periphery2,4, and abundance increases upon in vitro activation of CD4+CD25+ Tregs2. Additionally, expression of FOXP3 in naive T cells can convert these cells to a Treg phenotype4. FOXP3 is virtually undetectable in resting and activated CD4+CD25- T cells2. FOXP3 represents a more specific marker for CD4+ Tregs than e.g., CD25, CD45RB, CTLA-4, and GITR which do not discriminate between Tregs and activated, effector, or memory T cells4.

Additional Text

Concentration

0.5 mg/ml

Purification

Purified

Antibody Clonality

Monoclonal

Storage Note

This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Price

£46.00

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