Fibroblast Cell, 3T3/A31

Catalogue Number: F4209-USB

Manufacturer:	United States Biological
Physical state:	Supplied as a liquid in modified RIPA buffer with non-reducing sample buffer. Cellular Protein Preparation: Cells were lysed in modified RIPA buffer (50mM Tris HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM sodium chloride, 1mM EDTA, 1mM PMSF, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstatin, 1mM sodium vanadate, 1mM sodium fluoride). Diluted with non-reducing sample buffer (31mM Tris HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromophenol blue).
Type:	Cell Lysate
Shipping Condition:	Blue Ice
Unit(s):	50 ul, 100 ul
Application:	WB

FOR RESEARCH USE ONLY

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