Trans-Hi™ - In vitro Transfection Reagent

Catalogue Number: F90101TH-FOR

Manufacturer:	FormuMax
Type:	Transfection Reagents and Kit
Alias:	F90101TM, Transfection Reagent, Trans-Hi
Shipping Condition:	Blue Ice
Unit(s):	0.5 ml, 1 ml, 10 x 1 ml

Overview

Description

Description: Trans-Hi™ is a superior transfection reagent capable to compete against the leading products on the market. It is based on a proprietary polymer technology. It forms stable nanoparticles with DNA and rapidly releases the DNA molecules upon endocytosis into the cytoplasm. It is a broad spectrum transfection regent offering superior and reproducible transfection efficiency on the common cells such as Hela, CHO, HEK293 and many hard-to-transfect mammalian cells with minimal toxicity. Trans-Hi™ is very cost-efficient and 1.0mL is sufficient for over 660 transfections in 24 well plates. Key Features for Trans-HiTM Excellent transfection for single DNA transfection or multi DNA co-transfection Rapid onset and long-lasting transfection Suitable for very long DNAs Simple and easy to use transfection procedure Robust and reproducible transfection High level of recombinant protein production Very cost efficient Long shelf-life: 12 months Specifications: Protocols Trans-Hi™General Protocol For In Vitro Transfection in Mammalian Cells Trans-Hi™ - Protocol for Transfecting 293 and CHO Suspension Cells Trans-Hi™ - Protocol for Generation of rAAV Trans-Hi™ -Technical Tips For Good In Vitro Transfection Comparison study of In vitro plasmid DNA transfection of Trans-HiTM and Lipofectamine® 2000 in HEK293T cells with GFP vector using the manufacture recommended reagent/DNA ratio of 3:1 (uL/ug) and 0.5ug DNA per well used for both products in 24 well plate 30 hours post transfection. Trans-Hi™/DNA ratio (uL/ug) (HEK293T) Study of the transfection efficiency as a function of transfection reagent/DNA ratio indicating optimal tranfection efficiency achieved at Trans-Hi™/DNA ratio of 3:1 (uL/ug) (HEK293T cells in 24 well plate 30 hours post transfection, 0.5ug DNA per well). Head-to-head comparison Trans-Hi™ against Lipofectamine 2000 HEK293A cells with GFP vector (reagent/DNA ratio of 3:1(uL/ug, 0.5ug per well), 24 well plate 48 hours post transfection). Comparison study of In vitro plasmid DNA transfection of Trans-Hi™ and Lipofectamine® 2000 in Hela cells with GFP vector ((reagent/DNA ratio of 3:1(uL/ug, 0.5ug per well), 24 well plate 48 hours post transfection). Broad Transfection Spectrum for Mammalian Cell Types

Price

£172.00

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