Biotinylated Human Fc gamma RIIA/CD32a (R167) Protein (FCR-HM42AB)
Catalogue Number: FCR-HM42AB-KAT
FCR-HM42AB Image
FCR-HM42AB Image
FCR-HM42AB Image
FCR-HM42AB Image
FCR-HM42AB Image
The purity of Biotinylated Fc gamma RIIA (R167) is greater than 95% as determined by SEC-HPLC.
FCR-HM42AB Image
Biotinylated Human Fc gamma RIIA (R167) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
FCR-HM42AB Image
Biotinylated Human Fc gamma RIIA (R167) , His-Avi Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 2.17 uM as determined in SPR assay (Biacore T200) (QC Test).
FCR-HM42AB Image
Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIA (R167) , His-Avi Tag with an affinity constant of 1.03 uM as determined in SPR assay (Biacore T200).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Protein - Conjugated |
| Alias: | Fc gamma RIIA; FCG2; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; FCG2; CD32A; FcγRIIA; Fc γRIIA; Fcγ RIIA; Fc γ RIIA |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | WB |
Description
Description: The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
Additional Text
Protein Tag
HIS-AviTag™
Uniprot ID
P12318-1
Short Description
Recombinant Biotinylated Human Fc gamma RIIA/CD32a (R167) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala36-Ile218(R167).
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
The affinity constant of 1.03 OeM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
FCR-HM42AB Datasheet
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