Biotinylated Human Fc gamma RIIIA/CD16a (V176) Protein (FCR-HM43AB)
Catalogue Number: FCR-HM43AB-KAT
FCR-HM43AB Image
FCR-HM43AB Image
FCR-HM43AB Image
FCR-HM43AB Image
FCR-HM43AB Image
The purity of Biotinylated Human Fc gamma RIIIA (V176) is greater than 95% as determined by SEC-HPLC.
FCR-HM43AB Image
Biotinylated Human Fc gamma RIIIA (V176) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
FCR-HM43AB Image
Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIIA (V176) , His-Avi Tag with an affinity constant of 0.539 uM as determined in SPR assay (Biacore T200).
FCR-HM43AB Image
Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (V176) , His Tag with the EC50 of 9.8 ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Protein - Conjugated |
| Alias: | Fc gamma RIIIA; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20; FcγRIIIA; Fc γRIIIA; Fcγ RIIIA; Fc γ RIIIA |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: The Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Additional Text
Protein Tag
HIS-AviTag™
Accession Number
AAH17865
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Short Description
Recombinant Biotinylated Human Fc gamma RIIIA/CD16a (V176) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus; It contains Gly17-Gln208 (V176).
Biological Activity
Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1Oeg/ml (100Oel/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (V176) , His Tag with the EC50 of 2ng/ml determined by ELISA. The affinity constant of 0.539 OeM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
FCR-HM43AB Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations