Biotin Protein Labeling Kit, Protein Biotinylation Kit

Catalogue Number: FP-320-JEN

Manufacturer:	Jena Bioscience
Shelf Life:	12 months
Type:	Labelling/Staining Kit
Shipping Condition:	Blue Ice
Unit(s):	5 reactions

Overview
Datasheets

Description

Description: Binding of biotin by avidin, streptavidin or NeutrAvidin™ is the strongest known biological interaction with a dissociation constant in the range of 10-15 M. The vitamin biotin may be conjugated to many proteins without loss of their biological activity due to the small size of the biotin molecule. The biotinylated probe is usually detected by avidin, streptavidin or NeutrAvidin™, carrying a reporter group, e.g. horseradish peroxidase (HRP) or a fluorescent label. The protein of interest (POI) is often biotinylated at different positions. Each conjugated biotin binds one molecule of avidin, streptavidin or NeutrAvidin™, resulting in signal amplification. The interaction between biotin and biotin-binding proteins is used in various applications such as affinity chromatography, fluorescence-activated cell sorting (FACS), ELISA and Western Blot. This Protein Biotinylation Kit contains all reagents required for performing 5 separate labeling reactions of 5 mg of POI.

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FP-320 MSDS

FP-320 Datasheet

Price

£157.00

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