Goat anti Cat albumin

Catalogue Number: GACA/ALB-NMB

Manufacturer:	Nordic MuBio
Type:	Polyclonal Primary Antibody - Unconjugated
Shipping Condition:	RT
Unit(s):	1 ml
Host name:	Goat
Clone:
Isotype:
Immunogen:

Overview

Description

Description: The defined antibody specificity is directed to albumin as tested against cat sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against cat serum and the purified albumin. As precipitating antiserum to identify or measure cat albumin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.

Additional Text

Antibody Clonality

Polyclonal

Storage Note

The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Application Notes

Precipitation assays. In immunoelectrophoresis use 2 µl serum, plasma or equivalent against 120 µl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion and electroimmunodiffusion use 0.5 to 1.0 percent antiserum in the agar gel.

Price

£236.00

-

Quantity
Unit Size

Find out about delivery