Catalogue Number: GAHU/BJK(SD+HD)-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Polyclonal Secondary Antibody - Unconjugated |
| Shipping Condition: | RT |
| Unit(s): | 1 ml |
| Host name: | Goat |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Application: | IP |
Description: The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of human serum or plasma. To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immunodiffusion.
Polyclonal
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Precipitation assays. In immunoelectrophoresis use 2 µl serum or equivalent against 120 µl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.