Goat anti Monkey secretory component (free and bound)

Catalogue Number: GAMON/SC-NMB

Manufacturer:	Nordic MuBio
Type:	Polyclonal Secondary Antibody - Unconjugated
Shipping Condition:	RT
Unit(s):	1 ml
Host name:	Goat
Clone:
Isotype:
Immunogen:
Application:	IP

Overview

Description

Description: Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM.

Additional Text

Antibody Clonality

Polyclonal

Storage Note

The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Application Notes

Precipitation assays. In immunoelectrophoresis use 2 µl serum or equivalent against 120 µl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µl antiserum in 3 mm diameter centre well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in monkey secretions. In immunoelectrophoresis against monkey milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. The antiserum does not react with other molecular forms of IgA, or with any other secretory or plasma protein. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion to identify the presence secretory component in monkey serum or other body fluids and to determine its concentration.

Price

£236.00

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