Goat anti Swine haptoglobin

Catalogue Number: GASW/HP-NMB

Manufacturer:	Nordic MuBio
Type:	Polyclonal Primary Antibody - Unconjugated
Shipping Condition:	RT
Unit(s):	1 ml
Host name:	Goat
Clone:
Isotype:
Immunogen:
Application:	IP

Overview

Description

Description: The reactivity of the antiserum is restricted to haptoglobin. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal swine plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified haptoglobin. No reaction is obtained with any other plasma protein component or serum. In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of haptoglobin in swine plasma or other body fluids or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use in non-precipitating antibody-binding techniques if proper controls are included.

Additional Text

Gene Name

UniProt ID

Q8SPS7

Gene ID

397061

Antibody Clonality

Polyclonal

Storage Note

The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Application Notes

Precipitation assays. In immunoelectrophoresis use 2 ml or equivalent against 120 ml antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ml in a 3 mm diameter center well and 2 ml serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.

Price

£236.00

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