Human GDF15 Protein (GDF-HE115)
Catalogue Number: GDF-HE115-KAT
GDF-HE115 Image
GDF-HE115 Image
GDF-HE115 Image
GDF-HE115 Image
GDF-HE115 Image
Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).
GDF-HE115 Image
Human GDF15 on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.
GDF-HE115 Image
Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8 ng/ml.
GDF-HE115 Image
Immobilized Human GDF15, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 13.1 ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 13.5 kDa. The protein migrates to 15-16 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Other Recombinant Protein |
| Alias: | GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β |
| Host Cell: | E.coli |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.
Additional Text
Protein Tag
HIS Tag
Short Description
Recombinant Human GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ala197-Ile308.
UniProt ID
Q99988-1
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human GDF15, His Tag at 0.5Oeg/ml (100Oel/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 22.8ng/ml determined by ELISA. The affinity constant of 0.014 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
GDF-HE115 Datasheet
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