Human GFRAL/GFR alpha-like Protein (GFL-HM401)
Catalogue Number: GFL-HM401-KAT
GFL-HM401 Image
GFL-HM401 Image
GFL-HM401 Image
GFL-HM401 Image
GFL-HM401 Image
Human GFRAL on Bis-Tris PAGE under reduced condition.The purity is greater than 95%.
GFL-HM401 Image
Immobilized Human GFRAL, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human GDF15, hFc Tag with the EC50 of 3.3 ng/ml determined by ELISA.
GFL-HM401 Image
Loaded Human GDF15, hFc Tag on ProA-Biosensor can bind Human GFRAL, His-Avi Tag with an affinity constant of 35.50 nM as determined in BLI assay (Gator® Prime).
GFL-HM401 Image
The purity of-Human GFRAL is greater than 95% as determined by SEC-HPLC.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Other Recombinant Protein |
| Alias: | GFR alpha-like; GFRAL; GRAL; C6orf144 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain. GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
Additional Text
Gene ID
389400
Gene Name
GFRAL
Uniprot ID
Q6UXV0
Protein Tag
HIS-AviTag™
Short Description
Recombinant Human GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser19-Glu351.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human GFRAL, His Tag at 0.5Oeg/ml (100Oel/Well). Dose response curve for Human GDF15, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
GFL-HM401 Datasheet
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