Mouse GFRAL/GFR alpha-like Protein (GFL-MM401)
Catalogue Number: GFL-MM401-KAT
GFL-MM401 Image
GFL-MM401 Image
GFL-MM401 Image
GFL-MM401 Image
GFL-MM401 Image
Mouse GFRAL, His Tag immobilized on CM5 Chip can bind Mouse GDF15, His Tag with an affinity constant of 0.34 nM as determined in SPR assay (Biacore T200).
GFL-MM401 Image
The purity of Mouse GFRAL is greater than 95% as determined by SEC-HPLC.
GFL-MM401 Image
Mouse GFRAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
GFL-MM401 Image
Immobilized Mouse GFRAL, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Other Recombinant Protein |
| Alias: | GFR alpha-like; GFRAL; GRAL; C6orf144 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
Additional Text
Protein Tag
HIS-AviTag™
UniProt ID
Q6SJE0-1
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Short Description
Recombinant Mouse GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.; It contains Gln20-Glu350.
Biological Activity
Immobilized Mouse GFRAL, His Tag at 2Oeg/ml (100Oel/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. The affinity constant of 0.34 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
GFL-MM401 Datasheet
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