Human GM-CSF Protein (GSF-HE001)
Catalogue Number: GSF-HE001-KAT
GSF-HE001 Image
GSF-HE001 Image
GSF-HE001 Image
GSF-HE001 Image
GSF-HE001 Image
GSF-HE001 Image
Human GM-CSF R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human GM-CSF, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200).
GSF-HE001 Image
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.02-0.1 ng/mL (QC Test).
GSF-HE001 Image
The purity of Human GM-CSF is greater than 95% as determined by SEC-HPLC.
GSF-HE001 Image
Human GM-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
GSF-HE001 Image
Immobilized Human GM-CSF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 14.6 kDa same as Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Other Recombinant Protein |
| Alias: | CSF; CSF2; GMCSF; GM-CSF; MGC131935; MGC138897 |
| Host Cell: | E.coli |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim.
Additional Text
Gene Name
CSF2
Gene ID
1437
Uniprot ID
P04141
Protein Tag
Untagged
Short Description
Recombinant Human GM-CSF Protein is expressed from E.coli without tag.It contains Ala18-Glu144.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human GM-CSF at 2Oeg/ml (100Oel/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA. The affinity constant of 0.79nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 0.1 EU per µg by the LAL method.
GSF-HE001 Datasheet
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