Rat Cathepsin A protein, His and GST tag

Catalogue Number: GTX00059-PRO-GTX

Manufacturer:	GeneTex
Preservative:	ProClin™ 300
Physical state:	Lyophilized
Type:	Proteases
Alias:	cathepsin A , Ppgb
Host Cell:	E.coli
Shipping Condition:	Blue Ice
Unit(s):	10 ug
Application:	FA

Overview
Datasheets

Additional Text

Gene Name

CTSA

Protein Tag

GST Tag, HIS Tag

Gene ID

296370

Uniprot ID

Q6AYS3

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Application Notes

Cathepsin A (CTSA) is an enzyme that is classified both as a cathepsin and a carboxypeptidase. This gene encodes a glycoprotein that associates with lysosomal enzymes beta-galactosidase and neuraminidase to form a complex of high-molecular-weight multimers. The formation of this complex provides a protective role for stability and activity. It is protective for β-galactosidase and neuraminidase. Besides, Neuraminidase (NEU) has been identified as an interactor of CTSA, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CTSA and recombinant rat NEU. Briefly, CTSA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to NEU-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-CTSA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 µl stop solution to the wells and read at 450nm immediately. The binding activity of CTSA and NEU was in a dose dependent manner.

Storage Note

For short-term storage (1-2 weeks), store at 4C. For long-term storage, store at -20C or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

GTX00059-PRO Datasheet

Price

£312.00

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