Human MIP1 alpha protein, His tag (active)

Catalogue Number: GTX00096-PRO-GTX

Manufacturer:	GeneTex
Preservative:	ProClin™ 300
Physical state:	Lyophilized
Type:	Cytokines
Alias:	C-C motif chemokine ligand 3 , G0S19-1 , LD78ALPHA , MIP-1-alpha , MIP1A , SCYA3
Host Cell:	HEK293 cells
Shipping Condition:	Blue Ice
Unit(s):	10 ug
Application:	FA

Overview
Datasheets

Description

Description: This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010]

Additional Text

Gene ID

6348

Gene Name

CCL3

Uniprot ID

P10147

Accession Number

NP_002974.1

Protein Tag

HIS Tag

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Application Notes

MIP-1a (macrophage inflammatory protein 1-alpha) also known as Chemokine (C-C motief) ligand 3 (CCL3), is a cytokine belonging to the CC chemokine family that is involved in the recruitment and activation of macrophages, monocytes and neutrophils. In this case, chemotaxis assay used 24-well microchemotaxis system was undertaken to evaluate the chemotactic effect of MIP-1a on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100 µl cell suspension, 1x10⁶ cells/ml in RPMI-1640 with 0. 5% FBS) and MIP-1a (100 ng/ml, diluted in serum free RPMI-1640 ) was added in lower chamber with a polycarbonate filter (8µm pore size) used to separate the two compartments. After incubation at 37C with 5% CO₂ for 5h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (x40) and the number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter). By counting migrated cells in low chamber at high magnification (x400) randomly. A mean of 41. 2 THP-1 cells/field migrated towards serum free RPMI-1640 medium with 100 ng/ml MIP-1a, while only 3. 6 THP-1 cells/field migrated towards serum free RPMI-1640 medium.

Storage Note

For short-term storage (1-2 weeks), store at 4C. For long-term storage, store at -20C or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

GTX00096-PRO Datasheet

Price

£238.00

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