Human IL1 beta protein, His tag (active)

Catalogue Number: GTX00115-PRO-GTX

Manufacturer:	GeneTex
Preservative:	ProClin™ 300
Physical state:	Lyophilized
Type:	Interleukins
Alias:	interleukin 1 beta , IL-1 , IL1-BETA , IL1F2
Host Cell:	HEK293 cells
Shipping Condition:	Blue Ice
Unit(s):	10 ug
Application:	FA

Overview
Datasheets

Description

Description: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]

Additional Text

Gene Name

IL1B

Gene ID

3553

Uniprot ID

P01584

Accession Number

NP_000567.1

Protein Tag

HIS Tag

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Application Notes

Interleukin-1 beta belongs to the interleukin 1 cytokine family, which plays a critical role in inflammation, immunity, antiviral responses, and a variety of diseases. It has been reported that IL-1β-induced IL-6 production is mediated by both PI3K and IRAK4 in A549 cells. To test the bioactivity of IL-1β, A549 cells were seeded into 24-well plate at a density of 1x10⁵ cells/ml, and allowed to attach overnight before treated with or without certain concentrations (1 ng/ml, 10 ng/ml) of IL1-β for 4h. IL-6 levels in the cell supernatant were determined by ELISA that IL-6 increased significantly after stimulated with IL1-β.

Storage Note

For short-term storage (1-2 weeks), store at 4C. For long-term storage, store at -20C or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

GTX00115-PRO Datasheet

Price

£386.00

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