Human Osteopontin protein, His tag (active)

Catalogue Number: GTX00123-PRO-GTX

Manufacturer:	GeneTex
Preservative:	ProClin™ 300
Physical state:	Lyophilized
Type:	Cytokines
Alias:	secreted phosphoprotein 1 , BNSP , BSPI , ETA-1 , OPN
Host Cell:	HEK293 cells
Shipping Condition:	Blue Ice
Unit(s):	10 ug
Application:	FA

Overview
Datasheets

Description

Description: The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Additional Text

Uniprot ID

P10451

Gene Name

SPP1

Gene ID

6696

Accession Number

NP_000573.1

Protein Tag

HIS Tag

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Application Notes

Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which would promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappa B pathways in MCF-7 breast cancer cell line. Briefly, MCF-7 cells were seeded overnight at a density of 1x10⁵ cells/ml, and treated with or without OPN(100 ng/ml and 200 ng/ml) for 24h and MCP-1 levels in the cell supernatant were determined by ELISA. MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN.

Storage Note

For short-term storage (1-2 weeks), store at 4C. For long-term storage, store at -20C or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

GTX00123-PRO Datasheet

Price

£168.00

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