Human HMGB1 protein, His tag

Catalogue Number: GTX00154-PRO-GTX

Manufacturer:	GeneTex
Preservative:	ProClin™ 300
Physical state:	Lyophilized
Type:	DNA Binding Proteins
Alias:	high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1
Host Cell:	HEK293 cells
Shipping Condition:	Blue Ice
Unit(s):	10 ug
Application:	FA

Overview
Datasheets

Description

Description: This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]

Additional Text

Gene ID

3146

Gene Name

HMGB1

Uniprot ID

P09429

Protein Tag

HIS Tag

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Accession Number

NP_001300821.1

Application Notes

High Mobility Group Protein 1 (HMG1), also known as high mobility group box 1 proteinbelongs to high mobility group and contains HMG-box domain. HMG1 is one of the most important chromatin proteins. This nuclear protein organizes the DNA and regulates transcription. It supports transcription of many genes in interactions with many transcription factors. HMGB1 is secreted by immune cells (like macrophages, monocytes and dendritic cells) through leaderless secretory pathway. Activated macrophages and monocytes secrete HMGB1 as a cytokine mediator of Inflammation. Besides, Tumor Protein p53 (TP53) has been identified as an interactor of HMG1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human HMG1 and recombinant human TP53. Briefly, HMG1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to TP53-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-HMG1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 µl stop solution to the wells and read at 450nm immediately. The binding activity of HMG1 and TP53 was in a dose dependent manner.

Storage Note

For short-term storage (1-2 weeks), store at 4C. For long-term storage, store at -20C or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

GTX00154-PRO Datasheet

Price

£341.00

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