Human CD26 protein, His tag

Catalogue Number: GTX00187-PRO-GTX

Manufacturer:	GeneTex
Preservative:	ProClin™ 300
Physical state:	Lyophilized
Type:	Proteases
Alias:	dipeptidyl peptidase 4 , ADABP , ADCP2 , CD26 , DPPIV , TP103
Host Cell:	E.coli
Shipping Condition:	Blue Ice
Unit(s):	10 ug
Application:	FA

Overview
Datasheets

Description

Description: The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. [provided by RefSeq, Jul 2008]

Additional Text

Gene ID

1803

Gene Name

DPP4

Uniprot ID

P27487

Accession Number

NP_001926.2

Protein Tag

HIS Tag

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Application Notes

Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2 or cluster of differentiation 26 (CD26), is a protein in humans. DPP4 is an antigenic enzyme expressed on the surface of most cell types and is associated with immune regulation, signal transduction and apoptosis. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Besides, Eosinophil Chemotactic Factor (ECF) has been identified as an interactor of DPP4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human DPP4 and recombinant human ECF. Briefly, DPP4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to ECF-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-DPP4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 µl stop solution to the wells and read at 450nm immediately. The binding activity of DPP4 and ECF was in a dose dependent manner.

Storage Note

For short-term storage (1-2 weeks), store at 4C. For long-term storage, store at -20C or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

GTX00187-PRO Datasheet

Price

£238.00

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