Mouse TNF alpha protein, His tag (active)

Catalogue Number: GTX00288-PRO-GTX

Manufacturer:	GeneTex
Preservative:	ProClin™ 300
Physical state:	Lyophilized
Type:	Cytokines
Alias:	tumor necrosis factor , DIF , TNF-a , TNF-alpha , TNFSF2 , TNFalpha , Tnfa , Tnfsf1a , Tnlg1f
Host Cell:	HEK293 cells
Shipping Condition:	Blue Ice
Unit(s):	10 ug
Application:	FA

Overview
Datasheets

Description

Description: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Additional Text

Uniprot ID

P06804

Gene Name

TNF

Gene ID

21926

Protein Tag

HIS Tag

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Accession Number

NP_001265530.1

Application Notes

TNF-a (Tumor necrosis factor), being an endogenous pyrogen, is able to induce fever, necrosis, inflammation and to inhibit tumorigenesis. As reported, TNF-a could inhibit the proliferation and induce necrosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNFa (1 ng/ml, 10 ng/ml) for 2h, 4h, 8h, 24h, 48h, then cells were observed by inverted microscope. Cell necrosis after incubation with TNF-a (10 ng/ml) for 72h.

Storage Note

For short-term storage (1-2 weeks), store at 4C. For long-term storage, store at -20C or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

GTX00288-PRO Datasheet

Price

£238.00

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