NUP98 antibody [2H10]
Catalogue Number: GTX00697-GTX
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ICC/IF analysis of HeLa cells using GTX00697 NUP98 antibody [2H10]. Dilution : 0.5 µg/ml Fixation : Cold Methanol (-30 degree C) for 30 min
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ICC/IF analysis of S. pombe cells using GTX00697 NUP98 antibody [2H10]. Dilution : 10 µg/ml Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min Permeabilization : 1% Triton X-100 for 1 min
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ICC/IF analysis of S. cereviciae cells using GTX00697 NUP98 antibody [2H10]. Dilution : 10 µg/ml Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min Permeabilization : 1% Triton X-100 for 1 min
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ICC/IF analysis of HeLa cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. The signal at the nuclear periphery with 21A10 mAb was much higher and the background lower than that of 2H10 and 13C2 antibodies. Green : Primary antibody Violet : DAPI Dilution : 0.5 µg/ml Fixation : Cold Methanol (-30 degree C) for 30 min
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ICC/IF analysis of rat neuron cells using GTX00697 NUP98 antibody [2H10]. The dots are Nup98.
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Summary of the suitability of GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10] for immunological applications.
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WB analysis of S. pombe cell extracts expressing wilde type Nup98 (Lane 1) or Nup98-GFP protein (Lane 2) using GTX00697 NUP98 antibody [2H10]. Dilution : 1 µg/ml
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WB analysis of S. cereviciae cell extracts using GTX00697 NUP98 antibody [2H10]. Five protein bands identified by this antibody correspond to the sizes of 5 kinds of nucleoporins with multiple GFLG motifs in S. pombe. Dilution : 2 µg/ml
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WB analysis of HeLa nuclear membrane extract using GTX00697 NUP98 antibody [2H10]. Dilution : 1:2000
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Molecular Weight: | 198 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | nucleoporin 98 , ADIR2 , NUP196 , NUP96 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rat |
| Clone: | 2H10 |
| Isotype: | IgG2c |
| Immunogen: | Recombinant GST-fused human Nup98 (amino acids 1-466) |
| Application: | ELISA, ICC, IF, WB, DB |
Description
Description: Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin. [provided by RefSeq, Feb 2016]
Additional Text
Uniprot ID
P52948
Gene Name
NUP98
Gene ID
4928
Molecular Weight
198
Purification
Purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX00697 Datasheet
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