Haploid sperm cell-specific antigen antibody [TRA54]

Catalogue Number: GTX00716-GTX

Manufacturer:GeneTex
Preservative:no preservative|No preservative
Physical state:Liquid
Type:Monoclonal Primary Antibody - Unconjugated
Shipping Condition:Blue Ice
Unit(s): 100 ug
Host name: Rat
Clone: TRA54
Isotype: IgM
Immunogen: adult mouse testis
Application: ICC, IF, IHC-P, IP, EM, WB, IHC-Fr, DB

Additional Text

Purification

Purified

Antibody Clonality

Monoclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Concentration

1 mg/ml

Application Notes

WB: ~1 µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Short Description

Many transcripts for proteins specifically expressed in haploid germ cells are synthesized in the spermatogenesis. A monoclonal antibody TRA54 recognizes specific organelles in germ cell cytoplasm from spermatocytes to spermatids. Western blots using TRA54 revealed broad bands with 85 kD, 190 kD and >200 kDa in testis probably due to alternative splicing and/or post-translational modification. The molecular size diversity is due to the difference in glycosylation.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.