Rad51 antibody
Catalogue Number: GTX00721-GTX
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GTX00721 Image
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ICC/IF analysis of GM0637 cells irradiated by X-rays (2 Gy) for 1 hr using GTX00721 Rad51 antibody. Left : 1:100 dilution for 30 min at 37 degree C Right : 1:1000 for 30 min at 37 degree C Fixation : 4% PFA for 10 min Permeabilization : 0.5% Triton X-100 for 5 min
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SDS-PAGE analysis of the purified IgY.
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WB analysis of HeLa whole cell lysate using GTX00721 Rad51 antibody. Dilution : 1:1000
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Molecular Weight: | 37 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | RAD51 recombinase , BRCC5 , FANCR , HRAD51 , HsRad51 , HsT16930 , MRMV2 , RAD51A , RECA |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Chicken |
| Clone: | |
| Isotype: | IgY |
| Immunogen: | Recombinant full-size human red51 protein, no tag peptide attached. |
| Application: | ICC, IF, IP, WB |
Description
Description: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
Additional Text
Gene Name
RAD51
Uniprot ID
Q06609
Gene ID
5888
Molecular Weight
37
Purification
Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:1000-1:5000. ICC/IF: 1:100-1:2000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX00721 Datasheet
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