NUCB2 antibody
Catalogue Number: GTX00739-GTX
GTX00739 Image
GTX00739 Image
GTX00739 Image
GTX00739 Image
GTX00739 Image
ICC/IF analysis of P19 cells using GTX00739 NUCB2 antibody. Nucleobindin 2 was localized to the cytoplasm near the nucleus in undifferentiated P19 cells, and its immunoreactivity in the cytoplasm was increased when P19 cells were induced to differentiate into neurons. Left panel: undifferentiated murine embryonal carcinoma P19 cells (UD) Right panels: enriched post-mitotic neurons (PN)
GTX00739 Image
IHC-Fr analysis of cerebral cortex (parietal lobe) of mouse brain tissue section using GTX00739 NUCB2 antibody. At higher magnification (B, C), fine granular immunoreactive materials are observed at both neuronal dendrites (arrows) and perikarya (asterisks) in the layer IV (arrowhead B in A) and subplate (arrowhead C in A) of the cerebral cortex. Scale bars : 100 µm (A) and 10µm (B and C).
GTX00739 Image
WB analysis of various mouse tissue samples using GTX00739 NUCB2 antibody. Dilution : 1:1000
GTX00739 Image
WB analysis of mouse cerebral cortex using GTX00739 NUCB2 antibody. The apparent molecular mass (55~57 kDa) is larger than the calculated one (50 kDa) and the band is broad, which may reflect post-translational modifications. Dilution : 1:1000 Loading : 10 µg
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Molecular Weight: | 50 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | nucleobindin 2 , AI607786 , Calnuc , Nefa , Nesfatin-1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant GST-fused mouse nucleobindin 2 (aa 26-420) |
| Application: | AP, ICC, IF, IP, EM, WB, IHC-Fr |
Additional Text
Uniprot ID
P81117
Molecular Weight
50
Gene Name
Nucb2
Gene ID
53322
Purification
Protein A purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:1000-1:3000. ICC/IF: 1:300-1:1000. IHC-Fr: 1:300-1:1000. IP: 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX00739 Datasheet
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