NeuN antibody
Catalogue Number: GTX00837-GTX
GTX00837 Image
GTX00837 Image
GTX00837 Image
IHC (Free Floating) analysis of PFA-fixed rat hippocampus tissue using GTX00837 NeuN antibody. The NeuN antibody stains the nuclei and distal perikarya of most neurons, while the IBA1 antibody specifically labels microglial cells. Red : Primary antibody Green : IBA1 Dilution : 1:5,000
GTX00837 Image
WB analysis of mouse brain nuclear fraction lysate using GTX00837 NeuN antibody. Two bands at 46 and 50kDa mark correspond to the two isoforms of the FOX3/NeuN protein. Dilution : 1:1000
| Manufacturer: | GeneTex |
| Preservative: | 5 mM Sodium azide |
| Molecular Weight: | 34 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | FOX3 , HRNBP3 , NEUN , RBFOX3 , RNA binding protein, fox1 homolog 3 , NeuN , RNA binding fox-1 homolog 3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Chicken |
| Clone: | |
| Isotype: | IgY |
| Immunogen: | N-terminal 100 amino acids of human Fox3 expressed in and purified from E. coli |
| Application: | ICC, IF, IHC-P, WB, IHC-Fr, IHC, IHC-Flt |
Description
Description: This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]
Additional Text
Gene Name
RBFOX3
Uniprot ID
A6NFN3
Gene ID
146713
Purification
Unpurified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
34
Application Notes
WB: 1:500-1,000. ICC/IF: 1:5,000-10,000. IHC: 1:5,000-10,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX00837 Datasheet
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