ACE2 antibody [SN0754]
Catalogue Number: GTX01160-GTX
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IHC-P analysis of human kidney tissue section using GTX01160 ACE2 antibody [SN0754].
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Human tissue extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX01160 Image
ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human intestine. ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX01160 Image
ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX01160 Image
ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX01160 Image
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunofluorescent analysis. Sample: Vero E6 cells were fixed in ice-cold MeOH for 5 min. Green: ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10 µm.
GTX01160 Image
Untreated (-) and treated (+) Vero E6 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX01160 Image
Non-transfected (-) and transfected (+) Caco-2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | angiotensin I converting enzyme 2 , ACE2 , ACEH |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | SN0754 |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ACE2 aa 200-230. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]
Additional Text
Gene ID
59272
Uniprot ID
Q9BYF1
Concentration
1 mg/ml
Gene Name
ACE2
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
92
Application Notes
WB: 1:500~1:5000. ICC/IF: 1:100-1:500. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX01160 Datasheet
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