CD27 antibody [O323] (PE-Cy7)

Catalogue Number: GTX01457-10-GTX

Manufacturer:	GeneTex
Preservative:	0.09% sodium azide
Molecular Weight:	29
Physical state:	Liquid
Type:	Monoclonal Primary Antibody - Conjugated
Alias:	CD27 molecule , CD27 , S152 , S152. LPFS2 , T14 , TNFRSF7 , Tp55
Shipping Condition:	Blue Ice
Unit(s):	100 tests
Host name:	Mouse
Clone:	O323
Isotype:	IgG1
Immunogen:
Application:	FC

Overview
Datasheets

Description

Description: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]

Additional Text

Gene ID

939

Uniprot ID

P26842

Concentration

0.05 mg/mL

Purification

Affinity Purified

Gene Name

CD27

Antibody Clonality

Monoclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Short Description

The O323 antibody reacts with human CD27 (TNFRSF7), a cell surface homodimer of ≥ 55 kDa subunits, which provides co-stimulatory signaling in support of the T cell (TCR) and B cell (BCR) receptors. By comparison with CD28, whose TCR co-stimulatory signal can trigger cell proliferation, CD27 signaling appears to promote cell survival and differentiation to effector / memory stages. Also in contrast with CD28, the CD27 receptor may be shed following interaction with its ligand CD70, which is typically expressed on activated dendritic cells, T cells and B cells. With respect to B cells, CD27 is considered to be a phenotypic marker for memory B cells. CD27 has been included within a group of phenotypic markers for identifying human B regulatory cells (Bregs), a cell type proposed to regulate CD4+ T cell proliferation and Foxp3 / CTLA-4 expression in Treg cells. The O323 antibody may be used for analysis of CD27 expression on peripheral T cells, and is frequently used in combination with antibodies for IgD and IgM to distinguish naive vs. memory B cell populations. For identification of Breg cells, this antibody has been used in combination with antibodies for CD25, CD1d, IL-10 and TGF-beta. The antibody is also reported for cross-reactivity with Baboon, Cynomolgus and Rhesus CD27.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4C. DO NOT FREEZE. Protect from light.

Application Notes

FCM: 0.25 µg (5 µl) for 10⁵-10⁸ cells in 100 µl sample per test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

GTX01457-10 Datasheet

GTX01457-10 Reference

Price

£253.00

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