CD4 antibody [SK3] (PE-Cy7)

Catalogue Number: GTX01461-10-GTX

Manufacturer:	GeneTex
Preservative:	0.09% sodium azide
Molecular Weight:	51
Physical state:	Liquid
Type:	Monoclonal Primary Antibody - Conjugated
Alias:	CD4 molecule , CD4 , CD4mut
Shipping Condition:	Blue Ice
Unit(s):	100 tests
Host name:	Mouse
Clone:	SK3
Isotype:	IgG1
Immunogen:
Application:	FC

Overview
Datasheets

Description

Description: This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Additional Text

Gene ID

920

Uniprot ID

P01730

Purification

Affinity Purified

Gene Name

CD4

Antibody Clonality

Monoclonal

Concentration

0.012 mg/ml

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Short Description

The SK3 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells. The SK3 antibody is widely used as a phenotypic marker for human CD4 expression, and has been reported to be cross-reactive with Rhesus and Cynomolgus CD4. This antibody does not block binding of alternative clone RPA-T4, suggesting that they recognize different epitopes.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4C. DO NOT FREEZE. Protect from light.

Application Notes

FCM: 0.06 µg (5 µl) for 10⁵-10⁸ cells in 100 µl sample per test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

GTX01461-10 Datasheet

GTX01461-10 Reference

Price

£351.00

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