c-Kit antibody [ACK2] (APC-Cy7)
Catalogue Number: GTX01469-15-GTX
GTX01469-15 Image
GTX01469-15 Image
FACS analysis of mouse C57Bl/6 bone marrow cells using GTX01469-15 c-Kit antibody [ACK2] (APC-Cy7). Right panel : co-stained with c-Kit antibody [ACK2] (APC-Cy7) and Mouse CD45R antibody (violetFluor? 450) Left panel : co-stained with isotype control and Mouse CD45R antibody (violetFluor? 450) antibody amount : 0.25 µg (5 µl)
| Manufacturer: | GeneTex |
| Preservative: | 0.09% sodium azide |
| Molecular Weight: | 109 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rat |
| Clone: | ACK2 |
| Isotype: | IgG2b |
| Immunogen: | |
| Application: | FC |
Description
Description: The c-Kit proto-oncogene is the cellular homolog of the transforming gene of a feline retrovirus (v-Kit). The c-kit protein includes characteristics of a protein kinase transmembrane receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Additional Text
Gene ID
16590
Concentration
0.2 mg/ml
Purification
Affinity Purified
Uniprot ID
P05532
Gene Name
KIT
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
The ACK2 antibody is specific for CD117, also called c-Kit, a 145 kDa cytokine receptor important in the development of hematopoietic stem cells, in oogenesis, and for functional activity of immune cells such as NK and mast cells. c-Kit binds to a ligand known as stem cell factor (SCF), or alternatively as mast cell growth factor. Ligand binding promotes the activation (dimerization) and subsequent tyrosine kinase activity of the c-Kit receptor and triggers key survival, expansion and maturation signals during hematopoietic progenitor cell development. Conversely, shedding of extracellular domain of c-Kit receptor is reported to induce inactivation or apoptosis within these cells. The survival signaling activity of c-Kit confers a proto-oncogenic attribute to the receptor, as overexpression or mutations in this protein are associated with tumor development.The ACK2 antibody is widely utilized as a marker to identify hematopoietic progenitors, and to neutralize receptor-ligand binding in vitro and in vivo.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4C. DO NOT FREEZE. Protect from light.
GTX01469-15 Datasheet
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