IL2 Receptor alpha antibody [BC96] (PE-Cy7)
Catalogue Number: GTX01471-10-GTX
GTX01471-10 Image
GTX01471-10 Image
FACS analysis of human peripheral blood lymphocytes using GTX01471-10 IL2 Receptor alpha antibody [BC96] (PE-Cy7). Right panel : co-stained with IL2 Receptor alpha antibody [BC96] (PE-Cy7) and Human CD4 antibody (FITC) Left panel : co-stained with isotype control and Human CD4 antibody (FITC) antibody amount : 0.25 µg (5 µl)
| Manufacturer: | GeneTex |
| Preservative: | 0.09% sodium azide |
| Molecular Weight: | 31 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | interleukin 2 receptor subunit alpha , CD25 , IDDM10 , IL2R , IL2RA , IMD41 , TCGFR , p55 , IL2-R alpha |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 tests |
| Host name: | Mouse |
| Clone: | BC96 |
| Isotype: | IgG1 |
| Immunogen: | |
| Application: | FC |
Description
Description: The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Additional Text
Gene ID
3559
Uniprot ID
P01589
Concentration
0.05 mg/mL
Purification
Affinity Purified
Gene Name
IL2RA
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Short Description
The BC96 antibody is specific for human CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Signaling via CD25 may modulate Th17 and Treg cell differentiation, and has been shown to play a role in the function of dendritic cells. The BC96 antibody may be used as a marker for expression of CD25 during T and B cell development, on activated mature T and B cells, and for its expression at high levels on natural T regulatory cells (nTreg cells) in the thymus or periphery, and on "induced" peripheral Tregs. This antibody is cross-reactive with CD25 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4C. DO NOT FREEZE. Protect from light.
Application Notes
FCM: 0.25 µg (5 µl) for 10⁵-10⁸ cells in 100 µl sample per test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX01471-10 Datasheet
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