IL2 Receptor alpha antibody [PC61.5] (PE-Cy7)
Catalogue Number: GTX01472-10-GTX
GTX01472-10 Image
GTX01472-10 Image
FACS analysis of mouse C57Bl/6 splenocytes using GTX01472-10 IL2 Receptor alpha antibody [PC61.5] (PE-Cy7). Right panel : co-stained with IL2 Receptor alpha antibody [PC61.5] (PE-Cy7) and Mouse CD4 antibody (APC) Left panel : co-stained with isotype control and Mouse CD4 antibody (APC) antibody amount : 0.125 µg (5 µl)
| Manufacturer: | GeneTex |
| Preservative: | 0.09% sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rat |
| Clone: | PC61.5 |
| Isotype: | IgG1 |
| Immunogen: | |
| Application: | FC |
Additional Text
Gene ID
16184
Concentration
0.2 mg/ml
Purification
Affinity Purified
Gene Name
IL2RA
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
UniProt ID
P01590, Q544I2
Short Description
The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells. The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used to distinguish Treg cells from naive or conventional T cells which are CD25. This clone has also been reported for depletion of Treg cells in vivo.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4C. DO NOT FREEZE. Protect from light.
GTX01472-10 Datasheet
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