SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [CR3022]
Catalogue Number: GTX01555-GTX
GTX01555 Image
GTX01555 Image
GTX01555 Image
ELISA analysis of SARS-CoV-2 Spike S1 protein, Sheep Fc tag (blue line) and SARS-CoV-2 Spike S2 protein, Sheep Fc tag (orange line) at concentrations of 5 µg/ml using GTX01555 SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [CR3022]. A 3-fold serial dilution primary antibody from 125 ng/ml was performed. For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.
GTX01555 Image
ELISA analysis of SARS-CoV-2 Spike S1 protein, His tag (Insect Cells; grey line), SARS-CoV-2 Spike S2 protein, His tag (Insect Cells; yellow line) and SARS Coronavirus Spike S1 protein, His tag (HEK293 cells; blue line) at concentrations of 5 µg/ml using GTX01555 SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [CR3022]. A 3-fold serial dilution antibody from 41.6 ng/ml was performed. For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.
| Manufacturer: | GeneTex |
| Preservative: | 0.02% ProClin 300|0.02% ProClin 300 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | 2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Human |
| Clone: | CR3022 |
| Isotype: | IgG1 |
| Immunogen: | The original monoclonal antibody was generated by sequencing peripheral blood lymphocytes of a patient exposed to the SARS-CoV. |
| Application: | ELISA, NT, Inh |
Additional Text
Uniprot ID
P59594
Purification
Protein A purified
Gene ID
1489668
Gene Name
S
Antibody Clonality
Recombinant Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Short Description
SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [CR3022] binds to both SARS-CoV and SARS-CoV-2 with high affinity (PMID: 16796401 & 32065055). The initial characterization of the binding of this antibody was performed by ELISA and indicates potential for the development of diagnostic assays, as both virus-capture assays, or as controls in serological assays measuring immune responses to virus exposure. The original human IgG1 version of the antibody works synergistically in combination with another non-competing SARS antibody CR3014 and is a potential candidate for passive immune prophylaxis of SARS-CoV infection (ter Meulen et al., 2006). The original antibody (human IgG1) was also reported to bind the SARS-CoV-2 RBD (KD of 6.3 nM). This antibody may have potential as a therapeutic agent, alone or in combination with other neutralizing antibodies for treatment of SARS-CoV-2 infections (Tian et al., 2020).
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX01555 Datasheet
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