c-Fos antibody
Catalogue Number: GTX03375-GTX
GTX03375 Image
GTX03375 Image
GTX03375 Image
GTX03375 Image
ICC/IF analysis of HeLa cells were treated with PBS (A) or stimulated with 20% FBS (B) for 30 min after serum stravation for 36hr using GTX03375 c-Fos antibody. c-FOS antibody labels only the nuclei of stimulated cells. Red : Primary antibody Green : Tubulin Blue : DAPI Dilution : 1:2000
GTX03375 Image
IHC (Free Floating) analysis of Mouse hippocampus (C) or olfactory bulb (D) tissue sections using GTX03375 c-Fos antibody. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells. Red : Primary antibody Green : NF-L Blue : Hoechst staining of nuclear DNA Dilution : 1:10000
GTX03375 Image
WB analysis of various sample lysates using GTX03375 c-Fos antibody. Multiple bands at 50-65 kDa in stimulated or treated cell lysates, correspond to different isoforms of the c-FOS protein. Red : Primary antibody Green : GAPDH Dilution : 1:2000 Lane 1 : protein marker Lane 2 : HeLa cells grown in FBS free media Lane 3 : HeLa cells stimulated with 20% FBS for 2 hours after being in FBS free media for 36 hours Lane 4 : rat cortical neurons Lane 5 : rat cortical neurons treated with membrane depolarization buffer for 5 hours
| Manufacturer: | GeneTex |
| Preservative: | 5 mM Sodium azide |
| Molecular Weight: | 41 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Fos proto-oncogene, AP-1 transcription factor subunit , AP-1 , C-FOS , p55 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Full length recombinant human protein expressed in and purified from E. coli. |
| Application: | ICC, IF, WB, IHC-Flt |
Description
Description: The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
FOS
Gene ID
2353
Uniprot ID
P01100
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
41
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX03375 Datasheet
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