Mouse Annexin II protein, His and TrxA tag

Catalogue Number: GTX03782-PRO-GTX

Manufacturer:	GeneTex
Preservative:	no Preservative\|No Preservative
Physical state:	Lyophilized
Type:	Other Proteins
Alias:	annexin A2 , AW215814 , Cal1h
Host Cell:	E.coli
Shipping Condition:	Blue Ice
Unit(s):	10 ug
Application:	WB, FA

Overview
Datasheets

Additional Text

Gene Name

ANXA2

UniProt ID

P07356

Protein Tag

HIS Tag, TRX Tag

Gene ID

12306

Application Notes

Annexin A2 (ANXA2) also known as annexin II is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. It is a calcium-dependent phospholipid-binding protein whose function is to help organize exocytosis of intracellular proteins to the extracellular domain. Besides, S100 Calcium Binding Protein A10 (S100A10) has been identified as an interactor of ANXA2, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse ANXA2 and recombinant mouse S100A10. Briefly, ANXA2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to S100A10-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-ANXA2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 µl stop solution to the wells and read at 450nm immediately. The binding activity of ANXA2 and S100A10 was in a dose dependent manner.

Accession Number

NP_031611.1

Storage Note

For short-term storage (1-2 weeks), store at 4C. For long-term storage, store at -20C or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

GTX03782-PRO Datasheet

Price

£238.00

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