Mouse anti-Human IgM antibody [SA-DA4] (FITC)

Catalogue Number: GTX04300-06-GTX

Manufacturer:	GeneTex
Preservative:	0.09 % sodium azide\|0.09 % sodium azide
Physical state:	Liquid
Type:	Monoclonal Secondary Antibody - Conjugated
Shipping Condition:	Blue Ice
Unit(s):	500 ug
Host name:	Mouse
Clone:	SA-DA4
Isotype:	IgG1
Immunogen:	Human IgM myeloma protein
Application:	ELISA, FC, ICC, IF, IP, WB, IHC-Fr, Multiplex

Overview
Datasheets

Description

Description: Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010]

Additional Text

Concentration

0.5 mg/ml

Gene Name

IGHM

Gene ID

3507

Uniprot ID

P01871

Purification

Purified

Antibody Clonality

Monoclonal

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4C. Protect from light.

Application Notes

FCM: ≦1 µg/106cells in a final volume of 100 µL. ELISA: 1:200-1:400. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

GTX04300-06 Datasheet

Price

£386.00

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