GAPDH antibody

Catalogue Number: GTX100118-GTX

Manufacturer:GeneTex
Preservative:0.025% ProClin 300
Physical state:Liquid
Type:Polyclonal Primary Antibody - Unconjugated
Alias:glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP
Shipping Condition:Blue Ice
Unit(s): 100 ul, 25 ul
Host name: Rabbit
Clone:
Isotype: IgG
Immunogen: Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.
Application: ICC, IF, IHC-P, IP, WB

Description

Description: The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]

Additional Text

Gene Name

GAPDH

Concentration

0.11 mg/ml

Gene ID

2597

Purification

Affinity Purified

Uniprot ID

P04406

Antibody Clonality

Polyclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Molecular Weight

36

Application Notes

WB: 1:5000-1:100000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Short Description

GAPDH antibody recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein, a glycolytic enzyme of ~37 kDa that forms a tetramer. Because of its widespread expression, GAPDH is commonly used as an internal control for various protein assays, particularly western blot, to validate both the amount and quality of the loaded lysate samples.