PCNA antibody
Catalogue Number: GTX100539-GTX
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PCNA antibody detects PCNA protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract 12% SDS-PAGE PCNA antibody (GTX100539) dilution: 1:2500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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PCNA antibody detects PCNA protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12% SDS-PAGE PCNA antibody (GTX100539) dilution: 1:2500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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PCNA antibody detects PCNA protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PCNA antibody (GTX100539) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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PCNA antibody detects PCNA protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PCNA antibody (GTX100539) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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PCNA antibody detects PCNA protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. PCNA antibody (GTX100539) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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PCNA antibody detects PCNA protein at nucleus in human colon by immunohistochemical analysis. Sample: Paraffin-embedded human colon . PCNA antibody (GTX100539) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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PCNA antibody detects PCNA protein at nucleus in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. PCNA antibody (GTX100539) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 µg of PCBP2 antibody (GTX100539). Western blot analysis was performed using PCBP2 antibody (GTX100539). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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PCNA antibody detects PCNA protein by Western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (GTX100539) diluted at 1:2500.
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PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PCNA protein stained by PCNA antibody (GTX100539) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 µm.
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PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PCNA protein stained by PCNA antibody (GTX100539) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
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| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 29 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | proliferating cell nuclear antigen , ATLD2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB, IHC-Fr, IHC |
Description
Description: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq]
Additional Text
Gene Name
PCNA
Gene ID
5111
Uniprot ID
P12004
Concentration
0.11 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
29
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
PCNA antibody recognizes the proliferating cell nuclear antigen (PCNA), a cofactor of DNA polymerase delta with a predicted molecular weight of 29 kDa. PCNA is important for DNA synthesis, repair, and epigenetic modifications, and also serves as an organelle marker of the nucleus. PCNA antibody can be used for distinguishing between early, middle, and late S-phase of the cell cycle as well as grading of different neoplasms.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX100539 Datasheet
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