Nanog antibody [N3C3]
Catalogue Number: GTX100863-GTX
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Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using NANOG(GTX100863) antibody at 1:400 dilution.
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Immunoprecipitation of NANOG protein from NT2D1 whole cell extracts using 5 µg of NANOG antibody [N3C3] (GTX100863). Western blot analysis was performed using NANOG antibody [N3C3] (GTX100863) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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Sample (30 µg of whole cell lysate) A: HeLa nucleus 12% SDS PAGE GTX100863 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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ELISA detection of NANOG using GTX100863 for capture at a concentration of 5 µg/mL and GTX89079 for detection at a concentration of 5 µg/mL.
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The data was published in the journal Oncotarget in 2020.PMID: 32256979
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The data was published in the journal Nat Commun in 2018. PMID: 29973581
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The data was published in the journal J Biomed Sci in 2016.PMID: 27863490
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The data was published in the journal Elife in 2017.PMID: 28537559
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The data was published in the journal Elife in 2017.PMID: 28537559
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The data was published in the 2019 in Cancers (Basel). PMID: 31861872
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The data was published in the journal Cell Death Dis in 2015. PMID: 26136074
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The data was published in the journal Cell Death Dis in 2015. PMID: 26136074
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The data was published in the 2021 in Cancer Drug Resist. PMID: 35582384
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Nanog antibody [N3C3] detects Nanog protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 2% paraformaldehyde at RT for 15 min. Green: Nanog stained by Nanog antibody [N3C3] (GTX100863) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 35 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Nanog homeobox |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary. |
| Application: | ELISA, ICC, IF, IHC-P, IP, sELISA, WB, IHC-Fr |
Description
Description: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
Additional Text
Gene Name
NANOG
Concentration
0.5 mg/ml
Purification
Affinity Purified
Gene ID
79923
Uniprot ID
Q9H9S0
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
35
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. ELISA: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX100863 Datasheet
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