Annexin A1 antibody
Catalogue Number: GTX101070-GTX
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GTX101070 Image
GTX101070 Image
GTX101070 Image
GTX101070 Image
GTX101070 Image
GTX101070 Image
GTX101070 Image
GTX101070 Image
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Immunoprecipitation of Annexin A1 protein from HeLa whole cell extracts using 5 µg of Annexin A1 antibody (GTX101070). Western blot analysis was performed using Annexin A1 antibody (GTX101070). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX101070 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with Annexin A1 antibody (GTX101070) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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Annexin A1 antibody detects Annexin A1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Annexin A1 stained by Annexin A1 antibody (GTX101070) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10 µm.
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101070 Image
Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Annexin A1 antibody detects Annexin A1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Annexin A1 stained by Annexin A1 antibody (GTX101070) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101070 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101070 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
GTX101070 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
GTX101070 Image
Immunohistochemical analysis of paraffin-embedded N87 Xenograft , using Annexin I(GTX101070) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 39 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | annexin A1 , ANX1 , LPC1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Annexin A1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB |
Description
Description: Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq]
Additional Text
Gene Name
ANXA1
Uniprot ID
P04083
Gene ID
301
Molecular Weight
39
Concentration
1.33 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX101070 Datasheet
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