Heme Oxygenase 1 antibody
Catalogue Number: GTX101147-GTX
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Sandwich ELISA detection of recombinant full-length Heme Oxygenase 1 protein using GTX633677 as capture antibody at concentration of 5 µg/mL and GTX101147 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
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Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at endoplasmic reticulum and nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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The data was published in the journal Cell Death Dis in 2017.PMID: 28055010
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The data was published in the journal Int J Mol Sci in 2019. PMID: 30621167
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The data was published in the journal Cells in 2019. PMID: 31366086
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The data was published in the 2022 in Front Cell Neurosci. PMID: 35496911
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The data was published in the 2022 in Front Cell Neurosci. PMID: 35496911
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The data was published in the 2022 in Front Cell Neurosci. PMID: 35496911
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Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm on human renal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human renal carcinoma . Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
GTX101147 Image
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101147 Image
Untreated (-) and treated (+) HeLa whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 33 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | heme oxygenase 1 , HMOX1D , HO-1 , HSP32 , bK286B10 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary. |
| Application: | ELISA, ICC, IF, IHC-P, sELISA, WB |
Description
Description: Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq]
Additional Text
Gene Name
HMOX1
Uniprot ID
P09601
Concentration
0.54 mg/ml
Purification
Affinity Purified
Gene ID
3162
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
33
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX101147 Datasheet
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