MIF antibody [N1C3]
Catalogue Number: GTX101162-GTX
GTX101162 Image
GTX101162 Image
GTX101162 Image
GTX101162 Image
GTX101162 Image
GTX101162 Image
GTX101162 Image
GTX101162 Image
GTX101162 Image
Various tissue extracts (50 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (GTX101162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX101162 Image
Immunofluorescence analysis of methanol-fixed HeLa, using MIF(GTX101162) antibody at 1:200 dilution.
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Sample (30 µg of whole cell lysate) A: Molt-4 (GTX27912) 15% SDS PAGE GTX101162 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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ELISA detection of MIF using GTX101162 for capture at a concentration of 5 µg/mL and GTX89535 for detection at a concentration of 1.5 µg/mL.
GTX101162 Image
MIF antibody [N1C3] detects MIF protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MIF antibody [N1C3] (GTX101162) diluted at 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX101162 Image
MIF antibody [N1C3] detects MIF protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. MIF antibody [N1C3] (GTX101162) diluted at 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX101162 Image
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (GTX101162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101162 Image
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (GTX101162) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.01% Thimerosal |
| Molecular Weight: | 12 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | macrophage migration inhibitory factor , GIF , GLIF , MMIF |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human MIF. The exact sequence is proprietary. |
| Application: | ELISA, ICC, IF, IHC-P, sELISA, WB, IHC-Fr |
Description
Description: This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq]
Additional Text
Gene Name
MIF
Uniprot ID
P14174
Gene ID
4282
Concentration
0.1 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
12
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. ELISA: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX101162 Datasheet
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