HADHA antibody
Catalogue Number: GTX101177-GTX
GTX101177 Image
GTX101177 Image
GTX101177 Image
GTX101177 Image
GTX101177 Image
GTX101177 Image
GTX101177 Image
HADHA antibody detects HADHA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: HADHA protein stained by HADHA antibody (GTX101177) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
GTX101177 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HADHA antibody (GTX101177) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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HADHA antibody detects HADHA protein by Western blot analysis. A. 50 µg mouse kidney lysate/extract 7.5 % SDS-PAGE HADHA antibody (GTX101177) dilution: 1:500
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HADHA antibody detects HADHA protein by Western blot analysis. A.50 µg rat liver lysate/extract 7.5 % SDS-PAGE HADHA antibody (GTX101177) dilution: 1:500
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Sample(30 µg of whole cell lysate) A:Molt-4 (GTX27912) B:Raji(GTX27908) 7.5% SDS PAGE GTX101177 diluted at 1:1500
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Immunohistochemical analysis of paraffin-embedded human stomach , using HADHA(GTX101177) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 83 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha , ECHA , GBP , HADH , LCEH , LCHAD , MTPA , TP-ALPHA |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the C-terminus region of human HADHA. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq]
Additional Text
Gene Name
HADHA
Uniprot ID
P40939
Purification
Affinity Purified
Gene ID
3030
Molecular Weight
83
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX101177 Datasheet
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